Application
(LEFT)Western blot analysis of IL4 Antibody (C-term) in WiDr cell line lysates (35ug/lane).IL4 (arrow) was detected using the purified Pab.(RIGHT)Western blot analysis of IL4 Antibody (C-term) in mouse cerebellum cell line lysates (35ug/lane).IL4(arrow) was detected using the purified Pab.Application
IL4 Antibody (C-term) IHC analysis in formalin fixed and paraffin embedded tonsil followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the IL4 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.Application
IL4 Antibody (C-term) flow cytometric analysis of WiDr cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | IL4 Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | IL4; Interleukin-4; B-cell stimulatory factor 1; Binetrakin; Lymphocyte stimulatory factor 1; Pitrakinra |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | IL4 Interleukin-4 B-cell stimulatory factor 1 Binetrakin Lymphocyte stimulatory factor 1 Pitrakinra |
|---|---|
| Molecular Weight(MW) | 17492 Da |
| Function | Participates in at least several B-cell activation processes as well as of other cell types. It is a costimulator of DNA-synthesis. It induces the expression of class II MHC molecules on resting B-cells. It enhances both secretion and cell surface expression of IgE and IgG1. It also regulates the expression of the low affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes |
| Tissue Specificity | This L4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 130-160 amino acids from the C-terminal region of human L4. |
| Cellular Localization | Secreted. |
| Entrez Gene | 3565 |
|---|
Application
(LEFT)Western blot analysis of IL4 Antibody (C-term) in WiDr cell line lysates (35ug/lane).IL4 (arrow) was detected using the purified Pab.(RIGHT)Western blot analysis of IL4 Antibody (C-term) in mouse cerebellum cell line lysates (35ug/lane).IL4(arrow) was detected using the purified Pab.
Application
IL4 Antibody (C-term) IHC analysis in formalin fixed and paraffin embedded tonsil followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the IL4 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
IL4 Antibody (C-term) flow cytometric analysis of WiDr cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
