Application
Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with ILK Antibody (S246) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.Application
Western blot analysis of anti-ILK Antibody (S246) in Hela cell line lysates (35ug/lane). ILK(arrow) was detected using the purified Pab (1:60 dilution).Application
Western blot analysis of ILK (arrow) using rabbit polyclonal ILK Antibody (pS246) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the ILK gene.| Product Name | ILK Antibody (S246) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ILK; ILK1; ILK2; Integrin-linked protein kinase; 59 kDa serine/threonine-protein kinase; ILK-1; ILK-2; p59ILK |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| IHC:1:50~100 WB |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | ILK1 ILK2 |
|---|---|
| Alternative Names | ILK ILK1 ILK2 Integrin-linked protein kinase 59 kDa serine/threonine-protein kinase ILK-1 ILK-2 p59ILK |
| Molecular Weight(MW) | 51419 Da |
| Function | Receptor-proximal protein kinase regulating integrin- mediated signal transduction. May act as a mediator of inside-out integrin signaling. Focal adhesion protein part of the complex ILK-PINCH. This complex is considered to be one of the convergence points of integrin- and growth factor-signaling pathway. Could be implicated in mediating cell architecture, adhesion to integrin substrates and anchorage-dependent growth in epithelial cells Phosphorylates beta-1 and beta-3 integrin subunit on serine and threonine residues, but also AKT1 and GSK3B |
| Tissue Specificity | Highly expressed in heart followed by skeletal muscle, pancreas and kidney. Weakly expressed in placenta, lung and liver |
| Cellular Localization | Cell junction, focal adhesion. Cell membrane; Peripheral membrane protein; Cytoplasmic side. Cell projection, lamellipodium (By similarity). Cytoplasm, myofibril, sarcomere |
Application
Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with ILK Antibody (S246) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Application
Western blot analysis of anti-ILK Antibody (S246) in Hela cell line lysates (35ug/lane). ILK(arrow) was detected using the purified Pab (1:60 dilution).
Application
Western blot analysis of ILK (arrow) using rabbit polyclonal ILK Antibody (pS246) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the ILK gene.| Application Notes | IHC:1:50~100 WB |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
