Application
A. Western blot analysis on (A) HeLa, (B) 293T and (C) Jurkat cell lysates using anti-ILK RabMAb (Cat. #AJ1403b), dilution 1:50,000.Application
B. Immunofluorescent staining of HeLa cells using anti-ILK RabMAb (Cat. #AJ1403b).Application
C. Immunofluorescent staining of HeLa cells using anti-ILK RabMAb (Cat. #AJ1403b).| Product Name | ILK Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ILK, ILK1, ILK2, Integrin-linked protein kinase, ILK-1;ILK-2;59 kDa serine/threonine-protein kinase;p59ILK |
| Clonality | Monoclonal |
|---|---|
| Clone Number | EPR1592 |
| Host Species | Rabbit |
| Tested Applications | WBIF |
| WB:1:5000~10000 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | ILK1 ILK2 |
|---|---|
| Alternative Names | ILK ILK1 ILK2 Integrin-linked protein kinase ILK-1 ILK-2 59 kDa serine/threonine-protein kinase p59ILK |
| Molecular Weight(MW) | 51419 Da |
| Function | Receptor-proximal protein kinase regulating integrin- mediated signal transduction. May act as a mediator of inside-out integrin signaling. Focal adhesion protein part of the complex ILK-PINCH. This complex is considered to be one of the convergence points of integrin- and growth factor-signaling pathway. Could be implicated in mediating cell architecture, adhesion to integrin substrates and anchorage-dependent growth in epithelial cells Phosphorylates beta-1 and beta-3 integrin subunit on serine and threonine residues, but also AKT1 and GSK3B |
| Tissue Specificity | Highly expressed in heart followed by skeletal muscle, pancreas and kidney. Weakly expressed in placenta, lung and liver |
| Cellular Localization | Cell junction, focal adhesion. Cell membrane; Peripheral membrane protein; Cytoplasmic side. Cell projection, lamellipodium (By similarity). Cytoplasm, myofibril, sarcomere |
| Entrez Gene | 3611 |
|---|
Application
A. Western blot analysis on (A) HeLa, (B) 293T and (C) Jurkat cell lysates using anti-ILK RabMAb (Cat. #AJ1403b), dilution 1:50,000.
Application
B. Immunofluorescent staining of HeLa cells using anti-ILK RabMAb (Cat. #AJ1403b).
Application
C. Immunofluorescent staining of HeLa cells using anti-ILK RabMAb (Cat. #AJ1403b).| Application Notes | WB:1:5000~10000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
