Application
Western blot analysis of anti-Importin alpha-4 Pab in mouse heart tissue lysate (35ug/lane). Importin alpha-4(arrow) was detected using the purified Pab (1:50 dilution).| Product Name | Importin alpha-4 (KPNA4)Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | KPNA4; QIP1; Importin subunit alpha-4; Importin alpha Q1; Karyopherin subunit alpha-4 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:100~500 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | QIP1 |
|---|---|
| Alternative Names | KPNA4 QIP1 Importin subunit alpha-4 Importin alpha Q1 Karyopherin subunit alpha-4 |
| Molecular Weight(MW) | 57887 Da |
| Function | Functions in nuclear protein import as an adapter protein for nuclear receptor KPNB1. Binds specifically and directly to substrates containing either a simple or bipartite NLS motif. Docking of the importin/substrate complex to the nuclear pore complex (NPC) is mediated by KPNB1 through binding to nucleoporin FxFG repeats and the complex is subsequently translocated through the pore by an energy requiring, Ran- dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to importin-beta and the three components separate and importin-alpha and -beta are re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran from importin. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. In vitro, mediates the nuclear import of human cytomegalovirus UL84 by recognizing a non- classical NLS. In vitro, mediates the nuclear import of human cytomegalovirus UL84 by recognizing a non-classical NLS |
| Tissue Specificity | Highly expressed in testis, ovary, small intestine, heart, skeletal muscle, lung and pancreas, but barely detectable in kidney, thymus, colon and peripheral blood leukocytes |
| Cellular Localization | Cytoplasm (By similarity). Nucleus (By similarity) |
| Entrez Gene | 3840 |
|---|
Application
Western blot analysis of anti-Importin alpha-4 Pab in mouse heart tissue lysate (35ug/lane). Importin alpha-4(arrow) was detected using the purified Pab (1:50 dilution).| Application Notes | WB:1:100~500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
