Application
Formalin-fixed and paraffin-embedded human brain tissue reacted with INA antibody (Center I305) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.Application
Western blot analysis of INA (arrow) using rabbit polyclonal INA Antibody (Center) (AP6284e). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the INA gene (Lane 2) (Origene Technologies).Application
INA Antibody (Center I305) western blot analysis in HL-60 and MDA-MB453 cell line lysates (35ug/lane).This demonstrates the INA antibody detected the INA protein (arrow).| Product Name | INA Antibody (Center I305) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | INA; NEF5; Alpha-internexin; 66 kDa neurofilament protein; Neurofilament 5 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| IHC:1:50~100 WB |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | NEF5 |
|---|---|
| Alternative Names | INA NEF5 Alpha-internexin 66 kDa neurofilament protein Neurofilament 5 |
| Molecular Weight(MW) | 55391 Da |
| Function | Class-IV neuronal intermediate filament that is able to self-assemble. It is involved in the morphogenesis of neurons. It may form an independent structural network without the involvement of other neurofilaments or it may cooperate with NF-L to form the filamentous backbone to which NF-M and NF-H attach to form the cross-bridges |
| Tissue Specificity | Found predominantly in adult CNS. |
| Entrez Gene | 9118 |
|---|
Application
Formalin-fixed and paraffin-embedded human brain tissue reacted with INA antibody (Center I305) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Application
Western blot analysis of INA (arrow) using rabbit polyclonal INA Antibody (Center) (AP6284e). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the INA gene (Lane 2) (Origene Technologies).
Application
INA Antibody (Center I305) western blot analysis in HL-60 and MDA-MB453 cell line lysates (35ug/lane).This demonstrates the INA antibody detected the INA protein (arrow).| Application Notes | IHC:1:50~100 WB |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
