Application
Western blot analysis of INPP5K Antibody (Center) in A375 cell line lysates (35ug/lane). INPP5K (arrow) was detected using the purified Pab.Application
Formalin-fixed and paraffin-embedded human skeletal muscle reacted with INPP5K Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.Application
INPP5K Antibody (Center) FC analysis of ZR-75-1 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | INPP5K Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | INPP5K; PPS; SKIP; Inositol polyphosphate 5-phosphatase K; Skeletal muscle and kidney-enriched inositol phosphatase |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | PPS SKIP |
|---|---|
| Alternative Names | INPP5K PPS SKIP Inositol polyphosphate 5-phosphatase K Skeletal muscle and kidney-enriched inositol phosphatase |
| Molecular Weight(MW) | 51090 Da |
| Function | Inositol 5-phosphatase which acts on inositol 1,4,5- trisphosphate, inositol 1,3,4,5-tetrakisphosphate, phosphatidylinositol 4,5-bisphosphate and phosphatidylinositol 3,4,5-trisphosphate. Has 6-fold higher affinity for phosphatidylinositol 4,5-bisphosphate than for inositol 1,4,5- trisphosphate. May negatively regulate assembly of the actin cytoskeleton |
| Tissue Specificity | Ubiquitously expressed with highest levels in skeletal muscle, heart and kidney |
| Cellular Localization | Endoplasmic reticulum. Note=Following stimulation with EGF, translocates to membrane ruffles |
| Entrez Gene | 51763 |
|---|
Application
Western blot analysis of INPP5K Antibody (Center) in A375 cell line lysates (35ug/lane). INPP5K (arrow) was detected using the purified Pab.
Application
Formalin-fixed and paraffin-embedded human skeletal muscle reacted with INPP5K Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Application
INPP5K Antibody (Center) FC analysis of ZR-75-1 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
