Application
INS (Insulin) Antibody (Center) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the INS antibody detected the INS protein (arrow).Application
Confocal immunofluorescent analysis of INS (Insulin) Antibody (Center) with pancreas tissue followed by Alexa Fluor® 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).Application
INS Antibody (Insulin) (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human pancreas tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of INS Antibody (Insulin) (Center) for immunohistochemistry. Clinical relevance has not been evaluated.Application
INS (Insulin) Antibody (Center) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | INS (Insulin) Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | INS; Insulin; Insulin B chain; Insulin A chain |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIFIHCFC |
| WB:1:100~500 IF |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | INS Insulin Insulin B chain Insulin A chain |
|---|---|
| Molecular Weight(MW) | 11981 Da |
| Function | Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver |
| Tissue Specificity | This INS antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 42~72 amino acids from the Center region of human INS. |
| Cellular Localization | Secreted. |
| Entrez Gene | 3630 |
|---|
Application
INS (Insulin) Antibody (Center) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the INS antibody detected the INS protein (arrow).
Application
Confocal immunofluorescent analysis of INS (Insulin) Antibody (Center) with pancreas tissue followed by Alexa Fluor® 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Application
INS Antibody (Insulin) (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human pancreas tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of INS Antibody (Insulin) (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
INS (Insulin) Antibody (Center) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 IF |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
