Application
"Western blot analysis of anti-InsRR Pab in A375 cell lysate. Lane A: preimmune, Lane B: purified antibody. InsRR (Arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence."Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.| Product Name | Insulin Receptor R Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | INSRR; IRR; Insulin receptor-related protein; IR-related receptor; Insulin receptor-related protein alpha chain; Insulin receptor-related protein beta chain |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | IRR |
|---|---|
| Alternative Names | INSRR IRR Insulin receptor-related protein IR-related receptor Insulin receptor-related protein alpha chain Insulin receptor-related protein beta chain |
| Molecular Weight(MW) | 143720 Da |
| Function | Receptor with tyrosine-protein kinase activity. Functions as a pH sensing receptor which is activated by increased extracellular pH. Activates an intracellular signaling pathway that involves IRS1 and AKT1/PKB |
| Tissue Specificity | This Insulin Receptor R Antibody antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1265~1294 amino acids from the C-terminal region of human INSRR. |
| Cellular Localization | Membrane; Single-pass type I membrane protein |
| Entrez Gene | 3645 |
|---|
Application
"Western blot analysis of anti-InsRR Pab in A375 cell lysate. Lane A: preimmune, Lane B: purified antibody. InsRR (Arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence."
Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
