Application
Western blot analysis of anti-IPF Antibody (C-term) in HepG2 cell line lysates (35ug/lane). IPF(arrow) was detected using the purified Pab (1:60dilution).Application
Fluorescent confocal image of U251 cell stained with IPF Antibody (C-term)(Cat#AP7740b).U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with IPF primary antibody (1:25, 1 h at 37℃). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37℃).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). IPF immunoreactivity is localized to Cytoplasm and Nucleus significantly.| Product Name | IPF Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PDX1; IPF1; Pancreas/duodenum homeobox protein 1; Glucose-sensitive factor; Insulin promoter factor 1; Insulin upstream factor 1; Islet/duodenum homeobox-1; Somatostatin-transactivating factor 1 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIF |
| WB:1:50~100 IF |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | IPF1 STF1 |
|---|---|
| Alternative Names | PDX1 IPF1 Pancreas/duodenum homeobox protein 1 Glucose-sensitive factor Insulin promoter factor 1 Insulin upstream factor 1 Islet/duodenum homeobox-1 Somatostatin-transactivating factor 1 |
| Molecular Weight(MW) | 30771 Da |
| Function | Activates insulin, somatostatin, glucokinase, islet amyloid polypeptide and glucose transporter type 2 gene transcription. Particularly involved in glucose-dependent regulation of insulin gene transcription. As part of a PDX1:PBX1b:MEIS2b complex in pancreatic acinar cells is involved in the transcriptional activation of the ELA1 enhancer; the complex binds to the enhancer B element and cooperates with the transcription factor 1 complex (PTF1) bound to the enhancer A element. Binds preferentially the DNA motif 5'-[CT]TAAT[TG]-3' During development, specifies the early pancreatic epithelium, permitting its proliferation, branching and subsequent differentiation. At adult stage, required for maintaining the hormone-producing phenotype of the beta-cell |
| Tissue Specificity | Duodenum and pancreas (Langerhans islet beta cells and small subsets of endocrine non-beta-cells, at low levels in acinar cells) |
| Cellular Localization | Nucleus. Cytoplasm, cytosol (By similarity). |
| Entrez Gene | 3651 |
|---|
Application
Western blot analysis of anti-IPF Antibody (C-term) in HepG2 cell line lysates (35ug/lane). IPF(arrow) was detected using the purified Pab (1:60dilution).
Application
Fluorescent confocal image of U251 cell stained with IPF Antibody (C-term)(Cat#AP7740b).U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with IPF primary antibody (1:25, 1 h at 37℃). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37℃).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). IPF immunoreactivity is localized to Cytoplasm and Nucleus significantly.| Application Notes | WB:1:50~100 IF |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
