Application
A. Western blot analysis on Jurkat cell lysate using anti-IRAK-4 (N-term) RabMAb (Cat. #AJ1412a), 1:500 dilution.| Product Name | IRAK-4 Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | HEATR1, BAP28, HEAT repeat-containing protein 1, Protein BAP28 |
| Clonality | Monoclonal |
|---|---|
| Clone Number | Y279 |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500~2000 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | HEATR1 BAP28 HEAT repeat-containing protein 1 Protein BAP28 |
|---|---|
| Molecular Weight(MW) | 51530 Da |
| Function | Serine/threonine-protein kinase that plays a critical role in initiating innate immune response against foreign pathogens. Involved in Toll-like receptor (TLR) and IL-1R signaling pathways. Is rapidly recruited by MYD88 to the receptor- signaling complex upon TLR activation to form the Myddosome together with IRAK2. Phosphorylates initially IRAK1, thus stimulating the kinase activity and intensive autophosphorylation of IRAK1. Phosphorylates E3 ubiquitin ligases Pellino proteins (PELI1, PELI2 and PELI3) to promote pellino-mediated polyubiquitination of IRAK1. Then, the ubiquitin-binding domain of IKBKG/NEMO binds to polyubiquitinated IRAK1 bringing together the IRAK1-MAP3K7/TAK1-TRAF6 complex and the NEMO-IKKA-IKKB complex. In turn, MAP3K7/TAK1 activates IKKs (CHUK/IKKA and IKBKB/IKKB) leading to NF-kappa-B nuclear translocation and activation Alternatively, phosphorylates TIRAP to promote its ubiquitination and subsequent degradation. Phosphorylates NCF1 and regulates NADPH oxidase activation after LPS stimulation suggesting a similar mechanism during microbial infections |
| Tissue Specificity | A synthetic peptide corresponding to residues in the N-term of human IRAK-4 was used as immunogen. |
| Cellular Localization | Cytoplasm. |
| Entrez Gene | 51135 |
|---|
Application
A. Western blot analysis on Jurkat cell lysate using anti-IRAK-4 (N-term) RabMAb (Cat. #AJ1412a), 1:500 dilution.| Application Notes | WB:1:500~2000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
