Application
IREB2 Antibody (Center) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the IREB2 antibody detected the IREB2 protein (arrow).Application
IREB2 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of IREB2 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.Application
IREB2 Antibody (Center) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | IREB2 Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | IREB2; Iron-responsive element-binding protein 2; Iron regulatory protein 2 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | IREB2 Iron-responsive element-binding protein 2 Iron regulatory protein 2 |
|---|---|
| Molecular Weight(MW) | 105045 Da |
| Function | RNA-binding protein that binds to iron-responsive elements (IRES), which are stem-loop structures found in the 5'- UTR of ferritin, and delta aminolevulinic acid synthase mRNAs, and in the 3'-UTR of transferrin receptor mRNA. Binding to the IRE element in ferritin results in the repression of its mRNA translation. Binding of the protein to the transferrin receptor mRNA inhibits the degradation of this otherwise rapidly degraded mRNA |
| Tissue Specificity | This IREB2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 208-238 amino acids from the Central region of human IREB2. |
| Cellular Localization | Cytoplasm. |
| Entrez Gene | 3658 |
|---|---|
| Nucleotide Accession | NP_004127.1 |
Application
IREB2 Antibody (Center) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the IREB2 antibody detected the IREB2 protein (arrow).
Application
IREB2 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of IREB2 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
IREB2 Antibody (Center) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
