Application
A.Western blot analysis on MCF-7 cell lysates using anti-Phospho-IRF3 (pS386) RabMAb (Cat. #AJ1413c), 1:20,000 dilution. Cells were either (A) untreated (B) treated with Calyculin AApplication
B.Immunofluorescent staining of MCF7 cells using Phospho-specific anti-IRF3 RabMAb (Cat. #AJ1413c).| Product Name | IRF3 Antibody Phospho (pS386) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | IRF3, Interferon regulatory factor 3 |
| Modification | p-S386 |
| Clonality | Monoclonal |
|---|---|
| Clone Number | EPR2346 |
| Host Species | Rabbit |
| Tested Applications | WBIF |
| WB:1:2000~10000 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | IRF3 Interferon regulatory factor 3 |
|---|---|
| Molecular Weight(MW) | 47219 Da |
| Function | Key transcriptional regulator of type I interferon (IFN)-dependent immune responses and plays a critical role in the innate immune response against DNA and RNA viruses. Regulates the transcription of type I IFN genes (IFN-alpha and IFN-beta) and IFN-stimulated genes (ISG) by binding to an interferon-stimulated response element (ISRE) in their promoters. Acts as a more potent activator of the IFN-beta (IFNB) gene than the IFN-alpha (IFNA) gene and plays a critical role in both the early and late phases of the IFNA/B gene induction. Found in an inactive form in the cytoplasm of uninfected cells and following viral infection, double-stranded RNA (dsRNA), or toll-like receptor (TLR) signaling, becomes phosphorylated by IKBKE and TBK1 kinases. This induces a conformational change, leading to its dimerization and nuclear localization and association with CREB binding protein (CREBBP) to form dsRNA-activated factor 1 (DRAF1), a complex which activates the transcription of the type I IFN and ISG genes. Can activate distinct gene expression programs in macrophages and can induce significant apoptosis in primary macrophages |
| Tissue Specificity | Expressed constitutively in a variety of tissues |
| Cellular Localization | Cytoplasm. Nucleus. Note=Shuttles between cytoplasmic and nuclear compartments, with export being the prevailing effect. When activated, IRF3 interaction with CREBBP prevents its export to the cytoplasm |
| Entrez Gene | 3661 |
|---|
Application
A.Western blot analysis on MCF-7 cell lysates using anti-Phospho-IRF3 (pS386) RabMAb (Cat. #AJ1413c), 1:20,000 dilution. Cells were either (A) untreated (B) treated with Calyculin A
Application
B.Immunofluorescent staining of MCF7 cells using Phospho-specific anti-IRF3 RabMAb (Cat. #AJ1413c).| Application Notes | WB:1:2000~10000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
