Application
Western blot analysis of anti-IRF5 Antibody (N-term) in Ramos cell line lysates (35ug/lane). IRF5 (arrow) was detected using the purified Pab.Application
Confocal immunofluorescent analysis of IRF5 Antibody (N-term) with A549 cell followed by Alexa Fluor搴?489-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).Application
IRF5 Antibody (N-term) flow cytometry analysis of Ramos cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | IRF5 Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | IRF5; Interferon regulatory factor 5 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIFFC |
| WB:1:100~500 IF |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | IRF5 Interferon regulatory factor 5 |
|---|---|
| Molecular Weight(MW) | 56044 Da |
| Function | Transcription factor involved in the induction of interferons IFNA and INFB and inflammatory cytokines upon virus infection. Activated by TLR7 or TLR8 signaling |
| Tissue Specificity | This IRF5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 87~117 amino acids from the N-terminal region of human IRF5. |
| Cellular Localization | Cytoplasm. Nucleus. Note=Shuttles between the nucleus and the cytoplasm |
| Entrez Gene | 3663 |
|---|
Application
Western blot analysis of anti-IRF5 Antibody (N-term) in Ramos cell line lysates (35ug/lane). IRF5 (arrow) was detected using the purified Pab.
Application
Confocal immunofluorescent analysis of IRF5 Antibody (N-term) with A549 cell followed by Alexa Fluor搴?489-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Application
IRF5 Antibody (N-term) flow cytometry analysis of Ramos cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 IF |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
