Application
Western blot analysis of anti-IRF8 Antibody (Center) in Jurkat cell line lysates (35ug/lane). IRF8 (arrow) was detected using the purified Pab.Application
IRF8 Antibody (Center) flow cytometry analysis of Jurkat cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | IRF8 Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | IRF8; ICSBP1; Interferon regulatory factor 8; Interferon consensus sequence-binding protein |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBFC |
| WB:1:100~500 FC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | ICSBP1 |
|---|---|
| Alternative Names | IRF8 ICSBP1 Interferon regulatory factor 8 Interferon consensus sequence-binding protein |
| Molecular Weight(MW) | 48356 Da |
| Function | Specifically binds to the upstream regulatory region of type I IFN and IFN-inducible MHC class I genes (the interferon consensus sequence (ICS)). Plays a negative regulatory role in cells of the immune system. Involved in CD8(+) dendritic cell differentiation by forming a complex with the BATF-JUNB heterodimer in immune cells, leading to recognition of AICE sequence (5'-TGAnTCA/GAAA-3'), an immune-specific regulatory element, followed by cooperative binding of BATF and IRF8 and activation of genes (By similarity) |
| Tissue Specificity | Predominantly expressed in lymphoid tissues. |
| Cellular Localization | Nucleus. |
| Entrez Gene | 3394 |
|---|
Application
Western blot analysis of anti-IRF8 Antibody (Center) in Jurkat cell line lysates (35ug/lane). IRF8 (arrow) was detected using the purified Pab.
Application
IRF8 Antibody (Center) flow cytometry analysis of Jurkat cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 FC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
