Application
IRF9 Antibody (N-term) western blot analysis in mouse spleen tissue lysates (35ug/lane).This demonstrates the IRF9 antibody detected the IRF9 protein (arrow).Application
IRF9 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of IRF9 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.Application
Confocal immunofluorescent analysis of IRF9 Antibody (N-term)(Cat#AP11875a) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).| Product Name | IRF9 Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | IRF9; ISGF3G; Interferon regulatory factor 9; IFN-alpha-responsive transcription factor subunit; ISGF3 p48 subunit; Interferon-stimulated gene factor 3 gamma; Transcriptional regulator ISGF3 subunit gamma |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCIF |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | ISGF3G |
|---|---|
| Alternative Names | IRF9 ISGF3G Interferon regulatory factor 9 IFN-alpha-responsive transcription factor subunit ISGF3 p48 subunit Interferon-stimulated gene factor 3 gamma Transcriptional regulator ISGF3 subunit gamma |
| Molecular Weight(MW) | 43696 Da |
| Function | Transcription regulatory factor that mediates signaling by type I IFNs (IFN-alpha and IFN-beta). Following type I IFN binding to cell surface receptors, Jak kinases (TYK2 and JAK1) are activated, leading to tyrosine phosphorylation of STAT1 and STAT2 The phosphorylated STATs dimerize, associate with IRF9/ISGF3G to form a complex termed ISGF3 transcription factor, that enters the nucleus. ISGF3 binds to the IFN stimulated response element (ISRE) to activate the transcription of interferon stimulated genes, which drive the cell in an antiviral state |
| Tissue Specificity | This IRF9 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 82-112 amino acids from the N-terminal region of human IRF9. |
| Cellular Localization | Cytoplasm. Nucleus. Note=Translocated into the nucleus upon activation by IFN-alpha/beta |
Application
IRF9 Antibody (N-term) western blot analysis in mouse spleen tissue lysates (35ug/lane).This demonstrates the IRF9 antibody detected the IRF9 protein (arrow).
Application
IRF9 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of IRF9 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
Confocal immunofluorescent analysis of IRF9 Antibody (N-term)(Cat#AP11875a) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
