Application
IRGM Antibody (C-term) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the IRGM antibody detected the IRGM protein (arrow).Application
IRGM Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of IRGM Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.| Product Name | IRGM Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | IRGM; IFI1; IRGM1; LRG47; Immunity-related GTPase family M protein; Immunity-related GTPase family M protein 1; Interferon-inducible protein 1; LPS-stimulated RAW 264.7 macrophage protein 47 homolog |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | IFI1 IRGM1 LRG47 |
|---|---|
| Alternative Names | IRGM IFI1 IRGM1 LRG47 Immunity-related GTPase family M protein Immunity-related GTPase family M protein 1 Interferon-inducible protein 1 LPS-stimulated RAW 264.7 macrophage protein 47 homolog |
| Molecular Weight(MW) | 20142 Da |
| Function | Putative GTPase which is required for clearance of acute protozoan and bacterial infections. Functions in innate immune response probably through regulation of autophagy. May regulate proinflammatory cytokine production and prevent endotoxemia upon infection. May also play a role in macrophages adhesion and motility (By similarity) |
| Tissue Specificity | Widely expressed (at protein level). Expressed in several tissues including colon, small bowel and peripheral blood leukocytes |
| Cellular Localization | Golgi apparatus membrane (By similarity). Cell membrane (By similarity). Cytoplasmic vesicle, phagosome membrane (By similarity). Cytoplasmic vesicle, autophagosome membrane (By similarity). Cell projection, phagocytic cup (By similarity). Note=Behaves like an integral membrane protein (By similarity). Recruited to the plasma membrane around forming phagocytic cups, it remains associated with maturing autophagosomes (By similarity). Preferentially associated with cis- and medial-Golgi (By similarity) |
| Entrez Gene | 345611 |
|---|---|
| Nucleotide Accession | NP_001139277.1 |
Application
IRGM Antibody (C-term) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the IRGM antibody detected the IRGM protein (arrow).
Application
IRGM Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of IRGM Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
