Application
Western blot analysis of extracts from 293 cells treated withPMA (?M, 15min) or insulin(100nM, 30min) using IRS-1 Antibody (S639) (#AE1018b, Lane 1, 2 and 3) and Phospho-IRS-1-S639 Antibody (#AE1018d, Lane 4, 5 and 6).Application
Immunofluorescence staining of methanol-fixed HeLa cellsusing IRS-1 Antibody (S639) (#AE1018b, Red).| Product Name | IRS-1 Antibody (S639) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Insulin receptor substrate 1, IRS-1 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIF |
| WB:1:500~1:1000 IF |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | Insulin receptor substrate 1 IRS-1 |
|---|---|
| Molecular Weight(MW) | 131591 Da |
| Function | May mediate the control of various cellular processes by insulin. When phosphorylated by the insulin receptor binds specifically to various cellular proteins containing SH2 domains such as phosphatidylinositol 3-kinase p85 subunit or GRB2 Activates phosphatidylinositol 3-kinase when bound to the regulatory p85 subunit (By similarity) |
| Tissue Specificity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Entrez Gene | 3667 |
|---|
Application
Western blot analysis of extracts from 293 cells treated withPMA (?M, 15min) or insulin(100nM, 30min) using IRS-1 Antibody (S639) (#AE1018b, Lane 1, 2 and 3) and Phospho-IRS-1-S639 Antibody (#AE1018d, Lane 4, 5 and 6).
Application
Immunofluorescence staining of methanol-fixed HeLa cellsusing IRS-1 Antibody (S639) (#AE1018b, Red).| Application Notes | WB:1:500~1:1000 IF |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | affinity Purified IgG, in PBS, 0.02% sodium azide and 50% glycerol. |
