Application
A. Western blot analysis on 293 cell lysates using anti-Phospho-IRS-1 (pY896) RabMAb (Cat. #AJ1416b), 1:500,000 dilution. Cells were either (A) untreated (B) treated with insulin| Product Name | IRS-1 Antibody Phospho (pY896) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | IRS1, Insulin receptor substrate 1 |
| Modification | p-Y896 |
| Clonality | Monoclonal |
|---|---|
| Clone Number | EP260Y |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:1000~1:10000 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | IRS1 Insulin receptor substrate 1 |
|---|---|
| Molecular Weight(MW) | 131591 Da |
| Function | May mediate the control of various cellular processes by insulin. When phosphorylated by the insulin receptor binds specifically to various cellular proteins containing SH2 domains such as phosphatidylinositol 3-kinase p85 subunit or GRB2 Activates phosphatidylinositol 3-kinase when bound to the regulatory p85 subunit (By similarity) |
| Tissue Specificity | A synthetic peptide corresponding to residues near the C-terminus of human IRS-1was used as immunogen. |
| Entrez Gene | 3667 |
|---|
Application
A. Western blot analysis on 293 cell lysates using anti-Phospho-IRS-1 (pY896) RabMAb (Cat. #AJ1416b), 1:500,000 dilution. Cells were either (A) untreated (B) treated with insulin| Application Notes | WB:1:1000~1:10000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
