Application
ISWI Antibody (N-term) western blot analysis in SK-BR-3 cell line lysates (35ug/lane).This demonstrates the ISWI antibody detected the ISWI protein (arrow).| Product Name | ISWI Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | SMARCA5; SNF2H; WCRF135; SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 5; Sucrose nonfermenting protein 2 homolog |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:100~500 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | SNF2H WCRF135 |
|---|---|
| Alternative Names | SMARCA5 SNF2H WCRF135 SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 5 Sucrose nonfermenting protein 2 homolog |
| Molecular Weight(MW) | 121905 Da |
| Function | Helicase that possesses intrinsic ATP-dependent nucleosome-remodeling activity. Complexes containing SMARCA5 are capable of forming ordered nucleosome arrays on chromatin; this may require intact histone H4 tails. Also required for replication of pericentric heterochromatin in S-phase specifically in conjunction with BAZ1A. Probably plays a role in repression of polI dependent transcription of the rDNA locus, through the recruitment of the SIN3/HDAC1 corepressor complex to the rDNA promoter. Essential component of the WICH complex, a chromatin remodeling complex that mobilizes nucleosomes and reconfigures irregular chromatin to a regular nucleosomal array structure. The WICH complex regulates the transcription of various genes, has a role in RNA polymerase I and RNA polymerase III transcription, mediates the histone H2AX phosphorylation at 'Tyr-142', and is involved in the maintenance of chromatin structures during DNA replication processes. Essential component of the NoRC (nucleolar remodeling complex) complex, a complex that mediates silencing of a fraction of rDNA by recruiting histone-modifying enzymes and DNA methyltransferases, leading to heterochromatin formation and transcriptional silencing |
| Tissue Specificity | Ubiquitously expressed. |
| Cellular Localization | Nucleus. |
| Entrez Gene | 8467 |
|---|---|
| Nucleotide Accession | NP_003592.2 |
Application
ISWI Antibody (N-term) western blot analysis in SK-BR-3 cell line lysates (35ug/lane).This demonstrates the ISWI antibody detected the ISWI protein (arrow).| Application Notes | WB:1:100~500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
