Application
A. Western blot analysis on TF-1 cell lysate using anti-JAK3 RabMAb (Cat. #AJ1422b), dilution 1:2000| Product Name | JAK3 Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | JAK3, Tyrosine-protein kinase JAK3, Leukocyte janus kinase |
| Clonality | Monoclonal |
|---|---|
| Clone Number | EP909Y |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:2000 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | JAK3 Tyrosine-protein kinase JAK3 Leukocyte janus kinase |
|---|---|
| Molecular Weight(MW) | 125099 Da |
| Function | Non-receptor tyrosine kinase involved in various processes such as cell growth, development, or differentiation Mediates essential signaling events in both innate and adaptive immunity and plays a crucial role in hematopoiesis during T-cells development. In the cytoplasm, plays a pivotal role in signal transduction via its association with type I receptors sharing the common subunit gamma such as IL2R, IL4R, IL7R, IL9R, IL15R and IL21R. Following ligand binding to cell surface receptors, phosphorylates specific tyrosine residues on the cytoplasmic tails of the receptor, creating docking sites for STATs proteins Subsequently, phosphorylates the STATs proteins once they are recruited to the receptor. Phosphorylated STATs then form homodimer or heterodimers and translocate to the nucleus to activate gene transcription. For example, upon IL2R activation by IL2, JAK1 and JAK3 molecules bind to IL2R beta (IL2RB) and gamma chain (IL2RG) subunits inducing the tyrosine phosphorylation of both receptor subunits on their cytoplasmic domain. Then, STAT5A AND STAT5B are recruited, phosphorylated and activated by JAK1 and JAK3. Once activated, dimerized STAT5 translocates to the nucleus and promotes the transcription of specific target genes in a cytokine-specific fashion |
| Tissue Specificity | In NK cells and an NK-like cell line but not in resting T-cells or in other tissues. The S-form is more commonly seen in hematopoietic lines, whereas the B-form is detected in cells both of hematopoietic and epithelial origins |
| Cellular Localization | Endomembrane system; Peripheral membrane protein (By similarity). Cytoplasm (By similarity) |
| Entrez Gene | 3718 |
|---|
Application
A. Western blot analysis on TF-1 cell lysate using anti-JAK3 RabMAb (Cat. #AJ1422b), dilution 1:2000| Application Notes | WB:1:2000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
