Application
Western blot analysis of JAM3 Antibody (C-term) in Y79 cell line lysates (35ug/lane). JAM3 (arrow) was detected using the purified Pab.Application
Formalin-fixed and paraffin-embedded human brain tissue reacted with JAM3 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.Application
JAM3 Antibody (C-term) flow cytometric analysis of CEM cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | JAM3 Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB,IHC,FC |
| WB:1:50~100 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | JAM3 Junctional adhesion molecule C JAM-2 Junctional adhesion molecule 3 |
|---|---|
| Molecular Weight(MW) | 35020 Da |
| Function | Participates in cell-cell adhesion. It is a counter- receptor for ITGAM, mediating leukocyte-platelet interactions and is involved in the regulation of transepithelial migration of polymorphonuclear neutrophils (PMN). The soluble form is a mediator of angiogenesis |
| Tissue Specificity | Highest expression in placenta, brain and kidney. Significant expression is detected on platelets. Expressed in intestinal mucosa cells. Expressed in the vascular endothelium Found in serum (at protein level). Also detected in the synovial fluid of patients with rheumatoid arthritis, psoriatic arthritis or ostearthritis (at protein level) |
| Cellular Localization | Cell membrane; Single-pass type I membrane protein (Potential). Cell junction, desmosome. Secreted, extracellular space. Note=In epithelial cells, it is expressed at desmosomes but not at tight junctions. Localizes at the cell surface of endothelial cells; treatment of endothelial cells with vascular endothelial growth factor stimulates recruitment of JAM3 to cell-cell contacts |
| Entrez Gene | 83700 |
|---|
Application
Western blot analysis of JAM3 Antibody (C-term) in Y79 cell line lysates (35ug/lane). JAM3 (arrow) was detected using the purified Pab.
Application
Formalin-fixed and paraffin-embedded human brain tissue reacted with JAM3 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Application
JAM3 Antibody (C-term) flow cytometric analysis of CEM cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:50~100 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
