Application
Western blot analysis of TAOK3 (arrow) using rabbit polyclonal TAOK3 Antibody (Center) (Cat# AP7012c). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the TAOK3 gene (Lane 2) (Origene Technologies).Application
Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with TAOK3 antibody (Center) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.| Product Name | JIK (TAOK3) Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | TAOK3; DPK; JIK; KDS; MAP3K18; Serine/threonine-protein kinase TAO3; Cutaneous T-cell lymphoma-associated antigen HD-CL-09; Dendritic cell-derived protein kinase; JNK/SAPK-inhibitory kinase; Jun kinase-inhibitory kinase; Kinase from chicken homolog A; Thou |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:50~100 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | DPK JIK KDS MAP3K18 |
|---|---|
| Alternative Names | TAOK3 DPK JIK KDS MAP3K18 Serine/threonine-protein kinase TAO3 Cutaneous T-cell lymphoma-associated antigen HD-CL-09 Dendritic cell-derived protein kinase JNK/SAPK-inhibitory kinase Jun kinase-inhibitory kinase Kinase from chicken homolog A Thou |
| Molecular Weight(MW) | 105406 Da |
| Function | Serine/threonine-protein kinase that acts as a regulator of the p38/MAPK14 stress-activated MAPK cascade and of the MAPK8/JNK cascade. Acts as an activator of the p38/MAPK14 stress- activated MAPK cascade. In response to DNA damage, involved in the G2/M transition DNA damage checkpoint by activating the p38/MAPK14 stress-activated MAPK cascade, probably by mediating phosphorylation of upstream MAP2K3 and MAP2K6 kinases. Inhibits basal activity of MAPK8/JNK cascade and diminishes its activation in response epidermal growth factor (EGF) |
| Tissue Specificity | Ubiquitously expressed at a low level, and highly expressed in peripheral blood leukocytes (PBLs), thymus, spleen, kidney, skeletal muscle, heart and liver |
| Cellular Localization | Cytoplasm. Cell membrane; Peripheral membrane protein. Note=Also localized to the peripheral cell membrane |
| Entrez Gene | 51347 |
|---|
Application
Western blot analysis of TAOK3 (arrow) using rabbit polyclonal TAOK3 Antibody (Center) (Cat# AP7012c). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the TAOK3 gene (Lane 2) (Origene Technologies).
Application
Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with TAOK3 antibody (Center) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.| Application Notes | WB:1:50~100 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
