Application
Western blot analysis of JHDM1D Antibody (Center) in 293 cell line lysates (35ug/lane).JHDM1D (arrow) was detected using the purified Pab.Application
JHDM1D Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the JHDM1D Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.Application
JHDM1D Antibody (Center) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | JHDM1D Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | JHDM1D; KDM7; KIAA1718; Lysine-specific demethylase 7; JmjC domain-containing histone demethylation protein 1D |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | KDM7 KIAA1718 |
|---|---|
| Alternative Names | JHDM1D KDM7 KIAA1718 Lysine-specific demethylase 7 JmjC domain-containing histone demethylation protein 1D |
| Molecular Weight(MW) | 106557 Da |
| Function | Histone demethylase required for brain development. Specifically demethylates dimethylated 'Lys-9' and 'Lys-27' (H3K9me2 and H3K27me2, respectively) of histone H3 and monomethylated histone H4 'Lys-20' residue (H4K20Me1), thereby playing a central role in histone code. Specifically binds trimethylated 'Lys-4' of histone H3 (H3K4me3), affecting histone demethylase specificity: in presence of H3K4me3, it has no demethylase activity toward H3K9me2, while it has high activity toward H3K27me2. Demethylates H3K9me2 in absence of H3K4me3. Has activity toward H4K20Me1 only when nucleosome is used as a substrate and when not histone octamer is used as substrate |
| Tissue Specificity | This JHDM1D antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 295-325 amino acids from the Central region of human JHDM1D. |
| Cellular Localization | Nucleus. |
| Entrez Gene | 80853 |
|---|
Application
Western blot analysis of JHDM1D Antibody (Center) in 293 cell line lysates (35ug/lane).JHDM1D (arrow) was detected using the purified Pab.
Application
JHDM1D Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the JHDM1D Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
JHDM1D Antibody (Center) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
