Application
"Western blot analysis of SPAG9 (arrow) using rabbit polyclonal SPAG9 Antibody (Center) .293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the SPAG9 gene (Lane 2) (Origene Technologies)."Application
Formalin-fixed and paraffin-embedded human testis tissue reacted with SPAG9 antibody (Center) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.| Product Name | JLP (SPAG9) Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | SPAG9; HSS; KIAA0516; MAPK8IP4; SYD1; C-Jun-amino-terminal kinase-interacting protein 4; Cancer/testis antigen 89; Human lung cancer oncogene 6 protein; JNK-associated leucine-zipper protein; Mitogen-activated protein kinase 8-interacting protein 4; Prolif |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:50~100 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | HSS KIAA0516 MAPK8IP4 SYD1 |
|---|---|
| Alternative Names | SPAG9 HSS KIAA0516 MAPK8IP4 SYD1 C-Jun-amino-terminal kinase-interacting protein 4 Cancer/testis antigen 89 Human lung cancer oncogene 6 protein JNK-associated leucine-zipper protein Mitogen-activated protein kinase 8-interacting protein 4 Prolif |
| Molecular Weight(MW) | 146205 Da |
| Function | The JNK-interacting protein (JIP) group of scaffold proteins selectively mediates JNK signaling by aggregating specific components of the MAPK cascade to form a functional JNK signaling module. Isoform 5 may play a role in spermatozoa-egg- interaction |
| Tissue Specificity | Isoform 5 is expressed only in testis on the round spermatids of stage I, II and II. Isoform 5 is absent in spermatogonia and spermatocyte. Isoform 3 is expressed in testis Isoform 4 is expressed in testis and in acute myeloid leukemia (AML) patients |
| Cellular Localization | Cytoplasm. Cytoplasm, perinuclear region. Note=Perinuclear distribution in response to stress signals such as UV radiation |
| Entrez Gene | 9043 |
|---|
Application
"Western blot analysis of SPAG9 (arrow) using rabbit polyclonal SPAG9 Antibody (Center) .293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the SPAG9 gene (Lane 2) (Origene Technologies)."
Application
Formalin-fixed and paraffin-embedded human testis tissue reacted with SPAG9 antibody (Center) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.| Application Notes | WB:1:50~100 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
