Application
JHDM2a Antibody (C-term) western blot analysis in UM cell line lysates (35ug/lane).This demonstrates the JHDM2a antibody detected the JHDM2a protein (arrow).Application
JHDM2a Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of JHDM2a Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.| Product Name | JHDM2a Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | KDM3A; JHDM2A; JMJD1; JMJD1A; KIAA0742; TSGA; Lysine-specific demethylase 3A; JmjC domain-containing histone demethylation protein 2A; Jumonji domain-containing protein 1A |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | JHDM2A JMJD1 JMJD1A KIAA0742 TSGA |
|---|---|
| Alternative Names | KDM3A JHDM2A JMJD1 JMJD1A KIAA0742 TSGA Lysine-specific demethylase 3A JmjC domain-containing histone demethylation protein 2A Jumonji domain-containing protein 1A |
| Molecular Weight(MW) | 147341 Da |
| Function | Histone demethylase that specifically demethylates 'Lys- 9' of histone H3, thereby playing a central role in histone code Preferentially demethylates mono- and dimethylated H3 'Lys-9' residue, with a preference for dimethylated residue, while it has weak or no activity on trimethylated H3 'Lys-9'. Demethylation of Lys residue generates formaldehyde and succinate. Involved in hormone-dependent transcriptional activation, by participating in recruitment to androgen-receptor target genes, resulting in H3 'Lys-9' demethylation and transcriptional activation. Involved in spermatogenesis by regulating expression of target genes such as PRM1 and TMP1 which are required for packaging and condensation of sperm chromatin. Involved in obesity resistance through regulation of metabolic genes such as PPARA and UCP1 |
| Tissue Specificity | This JHDM2a antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 983-1017 amino acids from the C-terminal region of human JHDM2a. |
| Cellular Localization | Cytoplasm (By similarity). Nucleus (By similarity). Note=Nuclear in round spermatids. When spermatids start to elongate, localizes to the cytoplasm where it forms distinct foci which disappear in mature spermatozoa (By similarity) |
| Entrez Gene | 55818 |
|---|---|
| Nucleotide Accession | NP_001140160.1 NP_060903.2 |
Application
JHDM2a Antibody (C-term) western blot analysis in UM cell line lysates (35ug/lane).This demonstrates the JHDM2a antibody detected the JHDM2a protein (arrow).
Application
JHDM2a Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of JHDM2a Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
