Application
Western blot analysis of JMJD2B Antibody (N-term) in K562 cell line lysates (35ug/lane). JMJD2B (arrow) was detected using the purified Pab.Application
Formalin-fixed and paraffin-embedded human Testis tissue reacted with JMJD2B Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.Application
JMJD2B Antibody (N-term) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | JMJD2B Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | KDM4B; JHDM3B; JMJD2B; KIAA0876; Lysine-specific demethylase 4B; JmjC domain-containing histone demethylation protein 3B; Jumonji domain-containing protein 2B |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | JHDM3B JMJD2B KIAA0876 |
|---|---|
| Alternative Names | KDM4B JHDM3B JMJD2B KIAA0876 Lysine-specific demethylase 4B JmjC domain-containing histone demethylation protein 3B Jumonji domain-containing protein 2B |
| Molecular Weight(MW) | 121897 Da |
| Function | Histone demethylase that specifically demethylates 'Lys- 9' of histone H3, thereby playing a role in histone code. Does not demethylate histone H3 'Lys-4', H3 'Lys-27', H3 'Lys-36' nor H4 'Lys-20'. Only able to demethylate trimethylated H3 'Lys-9', with a weaker activity than KDM4A, KDM4C and KDM4D. Demethylation of Lys residue generates formaldehyde and succinate |
| Tissue Specificity | This JMJD2B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 10~40 amino acids from the N-terminal region of human JMJD2B. |
| Cellular Localization | Nucleus. |
| Entrez Gene | 23030 |
|---|
Application
Western blot analysis of JMJD2B Antibody (N-term) in K562 cell line lysates (35ug/lane). JMJD2B (arrow) was detected using the purified Pab.
Application
Formalin-fixed and paraffin-embedded human Testis tissue reacted with JMJD2B Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Application
JMJD2B Antibody (N-term) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
