Application
Western blot analysis of Phospho-JMJD2B-pT305 in mouse spleen tissue lysates (35ug/lane). JMJD2B (arrow) was detected using the purified Pab.Application
JMJD2B-T305 Antibody IHC analysis in formalin fixed and paraffin embedded mouse testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the JMJD2B-T305 Antibody for immunohistochemistry. Clinical relevance has not been evaluated.Application
JMJD2B-T305 Antibody flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | JMJD2B-T305 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | KDM4B; JHDM3B; JMJD2B; KIAA0876; Lysine-specific demethylase 4B; JmjC domain-containing histone demethylation protein 3B; Jumonji domain-containing protein 2B |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | JHDM3B JMJD2B KIAA0876 |
|---|---|
| Alternative Names | KDM4B JHDM3B JMJD2B KIAA0876 Lysine-specific demethylase 4B JmjC domain-containing histone demethylation protein 3B Jumonji domain-containing protein 2B |
| Molecular Weight(MW) | 121897 Da |
| Function | Histone demethylase that specifically demethylates 'Lys- 9' of histone H3, thereby playing a role in histone code. Does not demethylate histone H3 'Lys-4', H3 'Lys-27', H3 'Lys-36' nor H4 'Lys-20'. Only able to demethylate trimethylated H3 'Lys-9', with a weaker activity than KDM4A, KDM4C and KDM4D. Demethylation of Lys residue generates formaldehyde and succinate |
| Tissue Specificity | This JMJD2B-T305 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 287~316 amino acids from the T305 region of human JMJD2B. |
| Cellular Localization | Nucleus. |
| Entrez Gene | 23030 |
|---|
Application
Western blot analysis of Phospho-JMJD2B-pT305 in mouse spleen tissue lysates (35ug/lane). JMJD2B (arrow) was detected using the purified Pab.
Application
JMJD2B-T305 Antibody IHC analysis in formalin fixed and paraffin embedded mouse testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the JMJD2B-T305 Antibody for immunohistochemistry. Clinical relevance has not been evaluated.
Application
JMJD2B-T305 Antibody flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
