Application
Western blot analysis of anti-PTDSR (N-term) Pab (RB10820) in mouse heart, Hela, and HL60 cell line lysates. PTDSR (N-term)(arrow) was detected using the purified Pab.| Product Name | JMJD6 (PTDSR) Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Jmjd6; Ptdsr; Bifunctional arginine demethylase and lysyl-hydroxylase JMJD6; Histone arginine demethylase JMJD6; JmjC domain-containing protein 6; Jumonji domain-containing protein 6; Lysyl-hydroxylase JMJD6; Peptide-lysine 5-dioxygenase JMJD6; Phosphatidy |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:250~500 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | Ptdsr |
|---|---|
| Alternative Names | Jmjd6 Ptdsr Bifunctional arginine demethylase and lysyl-hydroxylase JMJD6 Histone arginine demethylase JMJD6 JmjC domain-containing protein 6 Jumonji domain-containing protein 6 Lysyl-hydroxylase JMJD6 Peptide-lysine 5-dioxygenase JMJD6 Phosphatidy |
| Molecular Weight(MW) | 46540 Da |
| Function | Dioxygenase that can both act as a histone arginine demethylase and a lysyl-hydroxylase. Acts as a lysyl-hydroxylase that catalyzes 5-hydroxylation on specific lysine residues of target proteins such as U2AF2/U2AF65 and LUC7L2. Acts as a regulator of RNA splicing by mediating 5-hydroxylation of U2AF2/U2AF65, affecting the pre-mRNA splicing activity of U2AF2/U2AF65. In addition to peptidyl-lysine 5-dioxygenase activity, may act as a RNA hydroxylase, as suggested by its ability to bind single strand RNA. Also acts as an arginine demethylase which demethylates histone H3 at 'Arg-2' (H3R2me) and histone H4 at 'Arg-3' (H4R3me), thereby playing a role in histone code. However, histone arginine demethylation may not constitute the primary activity in vivo. Has no histone lysine demethylase activity. Required for differentiation of multiple organs during embryogenesis. Acts as a key regulator of hematopoietic differentiation: required for angiogenic sprouting by regulating the pre-mRNA splicing activity of U2AF2/U2AF65. Seems to be necessary for the regulation of macrophage cytokine responses (By similarity) |
| Tissue Specificity | This JMJD6 (PTDSR) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1~30 amino acids from the N-terminal region of human PTDSR. |
| Cellular Localization | Nucleus, nucleoplasm (By similarity). Nucleus, nucleolus (By similarity). Note=Mainly found throughout the nucleoplasm outside of regions containing heterochromatic DNA, with some localization in nucleolus. During mitosis, excluded from the nucleus and reappears in the telophase of the cell cycle (By similarity) |
| Entrez Gene | 360665 |
|---|
Application
Western blot analysis of anti-PTDSR (N-term) Pab (RB10820) in mouse heart, Hela, and HL60 cell line lysates. PTDSR (N-term)(arrow) was detected using the purified Pab.| Application Notes | WB:1:250~500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
