Application
Western blot analysis of JMJD2A(arrow) using rabbit polyclonal JMJD2A (N-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the JMJD2A gene (Lane 2) (Origene Technologies).| Product Name | JMJD2A Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | KDM4A; JHDM3A; JMJD2; JMJD2A; KIAA0677; Lysine-specific demethylase 4A; JmjC domain-containing histone demethylation protein 3A; Jumonji domain-containing protein 2A |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:50~100 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | JHDM3A JMJD2 JMJD2A KIAA0677 |
|---|---|
| Alternative Names | KDM4A JHDM3A JMJD2 JMJD2A KIAA0677 Lysine-specific demethylase 4A JmjC domain-containing histone demethylation protein 3A Jumonji domain-containing protein 2A |
| Molecular Weight(MW) | 120662 Da |
| Function | Histone demethylase that specifically demethylates 'Lys- 9' and 'Lys-36' residues of histone H3, thereby playing a central role in histone code. Does not demethylate histone H3 'Lys-4', H3 'Lys-27' nor H4 'Lys-20'. Demethylates trimethylated H3 'Lys-9' and H3 'Lys-36' residue, while it has no activity on mono- and dimethylated residues. Demethylation of Lys residue generates formaldehyde and succinate. Participates in transcriptional repression of ASCL2 and E2F-responsive promoters via the recruitment of histone deacetylases and NCOR1, respectively |
| Tissue Specificity | Ubiquitous. |
| Cellular Localization | Nucleus. |
Application
Western blot analysis of JMJD2A(arrow) using rabbit polyclonal JMJD2A (N-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the JMJD2A gene (Lane 2) (Origene Technologies).| Application Notes | WB:1:50~100 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
