Application
Formalin-fixed and paraffin-embedded human brain tissue reacted with JTV1 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.Application
Western blot analysis of JTV1 Antibody (N-term) in T47D cell line lysates (35ug/lane). JTV1 (arrow) was detected using the purified Pab.Application
JTV1 Antibody (N-term) flow cytometric analysis of MCF-7 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Western blot analysis of JTV1 (arrow) using rabbit polyclonal JTV1 Antibody (N-term) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the JTV1 gene.| Product Name | JTV1 Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | AIMP2; JTV1; Aminoacyl tRNA synthase complex-interacting multifunctional protein 2; Multisynthase complex auxiliary component p38; Protein JTV-1 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHCWBFC |
| IHC:1:50~100 WB |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | JTV1 |
|---|---|
| Alternative Names | AIMP2 JTV1 Aminoacyl tRNA synthase complex-interacting multifunctional protein 2 Multisynthase complex auxiliary component p38 Protein JTV-1 |
| Molecular Weight(MW) | 35349 Da |
| Function | Required for assembly and stability of the aminoacyl- tRNA synthase complex. Mediates ubiquitination and degradation of FUBP1, a transcriptional activator of MYC, leading to MYC down- regulation which is required for aveolar type II cell differentiation. Blocks MDM2-mediated ubiquitination and degradation of p53/TP53. Functions as a proapoptotic factor |
| Tissue Specificity | This JTV1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 48~78 amino acids from the N-terminal region of human JTV1. |
| Cellular Localization | Cytoplasm, cytosol (By similarity). Nucleus (By similarity). Note=Following DNA damage, dissociates from the aminoacyl-tRNA synthase complex and translocates from the cytoplasm to the nucleus (By similarity) |
| Entrez Gene | 7965 |
|---|
Application
Formalin-fixed and paraffin-embedded human brain tissue reacted with JTV1 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Application
Western blot analysis of JTV1 Antibody (N-term) in T47D cell line lysates (35ug/lane). JTV1 (arrow) was detected using the purified Pab.
Application
JTV1 Antibody (N-term) flow cytometric analysis of MCF-7 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Western blot analysis of JTV1 (arrow) using rabbit polyclonal JTV1 Antibody (N-term) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the JTV1 gene.| Application Notes | IHC:1:50~100 WB |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
