Application
Formalin-fixed and paraffin-embedded human brain tissue reacted with JUN Antibody (S243) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.Application
Western blot analysis of JUN (arrow) using rabbit polyclonal JUN Antibody (S243) (Cat#AP1984g).293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the JUN gene (Lane 2) (Origene Technologies).| Product Name | JUN Antibody (T243) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | JUN; Transcription factor AP-1; Activator protein 1; Proto-oncogene c-Jun; V-jun avian sarcoma virus 17 oncogene homolog; p39 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| IHC:1:10~50 WB |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | JUN Transcription factor AP-1 Activator protein 1 Proto-oncogene c-Jun V-jun avian sarcoma virus 17 oncogene homolog p39 |
|---|---|
| Molecular Weight(MW) | 35676 Da |
| Function | Transcription factor that recognizes and binds to the enhancer heptamer motif 5'-TGA[CG]TCA-3'. Promotes activity of NR5A1 when phosphorylated by HIPK3 leading to increased steroidogenic gene expression upon cAMP signaling pathway stimulation |
| Tissue Specificity | This JUN antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide corresponding to amino acid residues surrounding T243 of human JUN. |
| Cellular Localization | Nucleus. |
| Entrez Gene | 3725 |
|---|
Application
Formalin-fixed and paraffin-embedded human brain tissue reacted with JUN Antibody (S243) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Application
Western blot analysis of JUN (arrow) using rabbit polyclonal JUN Antibody (S243) (Cat#AP1984g).293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the JUN gene (Lane 2) (Origene Technologies).| Application Notes | IHC:1:10~50 WB |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
