Application
Western blot analysis of JUN Antibody (C-term R272) in CEM cell line lysates (35ug/lane). JUN (arrow) was detected using the purified Pab.Application
JUN Antibody (C-term R272) flow cytometric analysis of CEM cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Confocal immunofluorescent analysis of JUN Antibody (C-term R272)(Cat#AP1984b) with 293 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).Application
JUN Antibody (C-term R272) immunohistochemistry analysis in formalin fixed and paraffin embedded human colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of JUN Antibody (C-term R272) for immunohistochemistry. Clinical relevance has not been evaluated.| Product Name | JUN Antibody (C-term R272) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | JUN; Transcription factor AP-1; Activator protein 1; Proto-oncogene c-Jun; V-jun avian sarcoma virus 17 oncogene homolog; p39 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBFCIFIHC |
| WB:1:50~100 FC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | JUN Transcription factor AP-1 Activator protein 1 Proto-oncogene c-Jun V-jun avian sarcoma virus 17 oncogene homolog p39 |
|---|---|
| Molecular Weight(MW) | 35676 Da |
| Function | Transcription factor that recognizes and binds to the enhancer heptamer motif 5'-TGA[CG]TCA-3'. Promotes activity of NR5A1 when phosphorylated by HIPK3 leading to increased steroidogenic gene expression upon cAMP signaling pathway stimulation |
| Tissue Specificity | This JUN antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 264~293 amino acids from the C-terminal(R272) region of human JUN. |
| Cellular Localization | Nucleus. |
| Entrez Gene | 3725 |
|---|
Application
Western blot analysis of JUN Antibody (C-term R272) in CEM cell line lysates (35ug/lane). JUN (arrow) was detected using the purified Pab.
Application
JUN Antibody (C-term R272) flow cytometric analysis of CEM cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Confocal immunofluorescent analysis of JUN Antibody (C-term R272)(Cat#AP1984b) with 293 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).
Application
JUN Antibody (C-term R272) immunohistochemistry analysis in formalin fixed and paraffin embedded human colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of JUN Antibody (C-term R272) for immunohistochemistry. Clinical relevance has not been evaluated.| Application Notes | WB:1:50~100 FC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
