Application
Western blot analysis of JUP Antibody (C-term) in MCF-7 cell line lysates (35ug/lane). JUP (arrow) was detected using the purified Pab.Application
Formalin-fixed and paraffin-embedded human lung carcinoma reacted with JUP Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.Application
JUP Antibody (C-term) flow cytometry analysis of MCF-7 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | JUP Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | JUP; CTNNG; DP3; Junction plakoglobin; Catenin gamma; Desmoplakin III; Desmoplakin-3 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | CTNNG DP3 |
|---|---|
| Alternative Names | JUP CTNNG DP3 Junction plakoglobin Catenin gamma Desmoplakin III Desmoplakin-3 |
| Molecular Weight(MW) | 81745 Da |
| Function | Common junctional plaque protein. The membrane- associated plaques are architectural elements in an important strategic position to influence the arrangement and function of both the cytoskeleton and the cells within the tissue. The presence of plakoglobin in both the desmosomes and in the intermediate junctions suggests that it plays a central role in the structure and function of submembranous plaques. Acts as a substrate for VE-PTP and is required by it to stimulate VE- cadherin function in endothelial cells. Can replace beta-catenin in E-cadherin/catenin adhesion complexes which are proposed to couple cadherins to the actin cytoskeleton (By similarity) |
| Tissue Specificity | This JUP antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 642~672 amino acids from the C-terminal region of human JUP. |
| Cellular Localization | Cell junction, adherens junction. Cell junction, desmosome. Cytoplasm, cytoskeleton. Membrane; Peripheral membrane protein. Note=Cytoplasmic in a soluble and membrane- associated form |
| Entrez Gene | 3728 |
|---|
Application
Western blot analysis of JUP Antibody (C-term) in MCF-7 cell line lysates (35ug/lane). JUP (arrow) was detected using the purified Pab.
Application
Formalin-fixed and paraffin-embedded human lung carcinoma reacted with JUP Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Application
JUP Antibody (C-term) flow cytometry analysis of MCF-7 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
