Application
Western blot analysis of K0776 Antibody (Center) in MCF-7 cell line lysates (35ug/lane). K0776 (arrow) was detected using the purified Pab.| Product Name | K0776 Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | UFL1; KIAA0776; NLBP; E3 UFM1-protein ligase 1; LZAP-binding protein |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:100~500 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | KIAA0776 NLBP |
|---|---|
| Alternative Names | UFL1 KIAA0776 NLBP E3 UFM1-protein ligase 1 LZAP-binding protein |
| Molecular Weight(MW) | 89595 Da |
| Function | E3 UFM1-protein ligase that mediates ufmylation of target proteins such as DDRGK1/C20orf116. The function of ufmylation is unknown. May act as a tumor suppressor by inhibiting cell invasion, blocking NF-kappa-B signaling and increasing stability of CDK5RAP3 |
| Tissue Specificity | Ubiquitously expressed, with a high expression in liver (at protein level). Low expression in several invasive hepatocellular carcinomas, such Hep-G2, Hep 3B2.1-7, HLE and PLC |
| Cellular Localization | Endoplasmic reticulum. Cytoplasm. Note=In cytoplasm colocalizes with CDK5RAP3 |
| Entrez Gene | 23376 |
|---|
Application
Western blot analysis of K0776 Antibody (Center) in MCF-7 cell line lysates (35ug/lane). K0776 (arrow) was detected using the purified Pab.| Application Notes | WB:1:100~500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
