Application
Western blot analysis of hHAT1-E404 in 293, HepG2, Jurkat cell line lysates (35ug/lane). HAT1 (arrow) was detected using the purified Pab.| Product Name | KAT1 (HAT1) Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | HAT1; KAT1; Histone acetyltransferase type B catalytic subunit; Histone acetyltransferase 1 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:50~100 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | KAT1 |
|---|---|
| Alternative Names | HAT1 KAT1 Histone acetyltransferase type B catalytic subunit Histone acetyltransferase 1 |
| Molecular Weight(MW) | 49513 Da |
| Function | Acetylates soluble but not nucleosomal histone H4 at 'Lys-5' (H4K5ac) and 'Lys-12' (H4K12ac) and, to a lesser extent, acetylates histone H2A at 'Lys-5' (H2AK5ac). Has intrinsic substrate specificity that modifies lysine in recognition sequence GXGKXG. May be involved in nucleosome assembly during DNA replication and repair as part of the histone H3.1 and H3.3 complexes. May play a role in DNA repair in response to free radical damage |
| Tissue Specificity | This KAT1 (HAT1) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 390~419 amino acids from the C-terminal region of human HAT1. |
| Cellular Localization | Isoform A: Nucleus matrix. |
| Entrez Gene | 8520 |
|---|
Application
Western blot analysis of hHAT1-E404 in 293, HepG2, Jurkat cell line lysates (35ug/lane). HAT1 (arrow) was detected using the purified Pab.| Application Notes | WB:1:50~100 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
