Application
Figure 1: Western blot analysis using anti-KDR monoclonal antibody against extracellular domain of human KDR (aa20-764).Application
Figure 2: Confocal immunofluorescence analysis of Hela (left) and HepG2 (right) cells using anti-KDR monoclonal antibody (green). Blue: DRAQ5 fluorescent DNA dye.Application
Figure 3: Flow cytometric analysis of HepG2 cells using anti-KDR mAb (green) and negative control (purple).| Product Name | KDR Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | FLK1; CD309; VEGFR; VEGFR2 |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 4B4 |
| Host Species | Mouse |
| Tested Applications | WBIFFC |
| WB:1:500 - 2000. IF |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | FLK1 VEGFR2 |
|---|---|
| Alternative Names | FLK1 CD309 VEGFR VEGFR2 |
| Molecular Weight(MW) | 151527 Da |
| Function | Tyrosine-protein kinase that acts as a cell-surface receptor for VEGFA, VEGFC and VEGFD. Plays an essential role in the regulation of angiogenesis, vascular development, vascular permeability, and embryonic hematopoiesis. Promotes proliferation, survival, migration and differentiation of endothelial cells Promotes reorganization of the actin cytoskeleton. Isoforms lacking a transmembrane domain, such as isoform 2 and isoform 3, may function as decoy receptors for VEGFA, VEGFC and/or VEGFD Isoform 2 plays an important role as negative regulator of VEGFA- and VEGFC-mediated lymphangiogenesis by limiting the amount of free VEGFA and/or VEGFC and preventing their binding to FLT4 Modulates FLT1 and FLT4 signaling by forming heterodimers. Binding of vascular growth factors to isoform 1 leads to the activation of several signaling cascades. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate and the activation of protein kinase C. Mediates activation of MAPK1/ERK2, MAPK3/ERK1 and the MAP kinase signaling pathway, as well as of the AKT1 signaling pathway. Mediates phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, reorganization of the actin cytoskeleton and activation of PTK2/FAK1. Required for VEGFA-mediated induction of NOS2 and NOS3, leading to the production of the signaling molecule nitric oxide (NO) by endothelial cells. Phosphorylates PLCG1. Promotes phosphorylation of FYN, NCK1, NOS3, PIK3R1, PTK2/FAK1 and SRC |
| Tissue Specificity | Detected in cornea (at protein level). Widely expressed |
| Cellular Localization | Isoform 1: Cell membrane; Single-pass type I membrane protein. Cytoplasm. Nucleus. Cytoplasmic vesicle. Early endosome. Note=Detected on caveolae-enriched lipid rafts at the cell surface. Is recycled from the plasma membrane to endosomes and back again. Phosphorylation triggered by VEGFA binding promotes internalization and subsequent degradation. VEGFA binding triggers internalization and translocation to the nucleus Isoform 3: Secreted. |
| Entrez Gene | 3791 |
|---|---|
| Nucleotide Accession | NP_002244.1 |
Application
Figure 1: Western blot analysis using anti-KDR monoclonal antibody against extracellular domain of human KDR (aa20-764).
Application
Figure 2: Confocal immunofluorescence analysis of Hela (left) and HepG2 (right) cells using anti-KDR monoclonal antibody (green). Blue: DRAQ5 fluorescent DNA dye.
Application
Figure 3: Flow cytometric analysis of HepG2 cells using anti-KDR mAb (green) and negative control (purple).| Application Notes | WB:1:500 - 2000. IF |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified recombinant extracellular fragment of human KDR (aa20-764) fused with hIgGFc tag expressed in HEK293 cells. |
