Application
KHSRP Antibody (Center) western blot analysis in U251 cell line lysates (35ug/lane).This demonstrates the KHSRP antibody detected the KHSRP protein (arrow).Application
KHSRP Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of KHSRP Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.Application
Confocal immunofluorescent analysis of KHSRP Antibody (Center)(Cat#AP11709c) with U-251MG cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).| Product Name | KHSRP Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | KHSRP; FUBP2; Far upstream element-binding protein 2; KH type-splicing regulatory protein; p75 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCIF |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | FUBP2 |
|---|---|
| Alternative Names | KHSRP FUBP2 Far upstream element-binding protein 2 KH type-splicing regulatory protein p75 |
| Molecular Weight(MW) | 73115 Da |
| Function | Binds to the dendritic targeting element and may play a role in mRNA trafficking (By similarity). Part of a ternary complex that binds to the downstream control sequence (DCS) of the pre-mRNA. Mediates exon inclusion in transcripts that are subject to tissue-specific alternative splicing. May interact with single- stranded DNA from the far-upstream element (FUSE). May activate gene expression. Also involved in degradation of inherently unstable mRNAs that contain AU-rich elements (AREs) in their 3'- UTR, possibly by recruiting degradation machinery to ARE- containing mRNAs |
| Tissue Specificity | Detected in neural and non-neural cell lines. |
| Cellular Localization | Nucleus. Cytoplasm. Note=A small proportion is also found in the cytoplasm of neuronal cell bodies and dendrites (By similarity) |
| Entrez Gene | 8570 |
|---|---|
| Nucleotide Accession | NP_003676.2 |
Application
KHSRP Antibody (Center) western blot analysis in U251 cell line lysates (35ug/lane).This demonstrates the KHSRP antibody detected the KHSRP protein (arrow).
Application
KHSRP Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of KHSRP Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
Confocal immunofluorescent analysis of KHSRP Antibody (Center)(Cat#AP11709c) with U-251MG cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
