Application
A.Immunohistochemical analysis of paraffin-embedded human tonsils using anti-Ki-67 RabMAb. Note nuclear staining of proliferating cells (Cat. #AJ1427a).Application
B. Immunofluorescent staining of HeLa cells using anti-Ki-67 RabMAb (Cat. #AJ1427a).| Product Name | Ki-67 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | MKI67, Antigen KI-67 |
| Clonality | Monoclonal |
|---|---|
| Clone Number | EPR3611 |
| Host Species | Rabbit |
| Tested Applications | IHCIF |
| IHC:1:500~1000 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | MKI67 Antigen KI-67 |
|---|---|
| Molecular Weight(MW) | 358694 Da |
| Function | Thought to be required for maintaining cell proliferation |
| Tissue Specificity | A synthetic peptide corresponding to residues in human Ki-67 was used as an immunogen. |
| Cellular Localization | Nucleus. Nucleus, nucleolus. Chromosome. Note=Predominantly localized in the G1 phase in the perinucleolar region, in the later phases it is also detected throughout the nuclear interior, being predominantly localized in the nuclear matrix. In mitosis, it is present on all chromosomes |
| Entrez Gene | 4288 |
|---|
Application
A.Immunohistochemical analysis of paraffin-embedded human tonsils using anti-Ki-67 RabMAb. Note nuclear staining of proliferating cells (Cat. #AJ1427a).
Application
B. Immunofluorescent staining of HeLa cells using anti-Ki-67 RabMAb (Cat. #AJ1427a).| Application Notes | IHC:1:500~1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
