Application
Western blot analysis of KIAA0174 Antibody (N-term) in A2058 cell line lysates (35ug/lane). KIAA0174 (arrow) was detected using the purified Pab.Application
KIAA0174 Antibody (N-term) IHC analysis in formalin fixed and paraffin embedded lung tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the KIAA0174 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.Application
KIAA0174 Antibody (N-term) flow cytometric analysis of A2058 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | KIAA0174 Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | IST1; KIAA0174; IST1 homolog; Putative MAPK-activating protein PM28 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | KIAA0174 |
|---|---|
| Alternative Names | IST1 KIAA0174 IST1 homolog Putative MAPK-activating protein PM28 |
| Molecular Weight(MW) | 39751 Da |
| Function | Proposed to be involved in specific functions of the ESCRT machinery. Is required for efficient abscission during cytokinesis, but not for HIV-1 budding. The involvement in the MVB pathway is not established. Involved in recruiting VPS4A and/or VPS4B to the midbody of dividing cells |
| Tissue Specificity | This KIAA0174 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 21~50 amino acids from the N-terminal region of human KIAA0174. |
| Cellular Localization | Cytoplasmic vesicle. Note=Localizes to the midbody of dividing cells |
| Entrez Gene | 9798 |
|---|
Application
Western blot analysis of KIAA0174 Antibody (N-term) in A2058 cell line lysates (35ug/lane). KIAA0174 (arrow) was detected using the purified Pab.
Application
KIAA0174 Antibody (N-term) IHC analysis in formalin fixed and paraffin embedded lung tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the KIAA0174 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
KIAA0174 Antibody (N-term) flow cytometric analysis of A2058 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
