Application
Western blot analysis of anti-KIS Pab in mouse heart tissue lysate. KIS (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.Application
KIS Antibody (C6) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the KIS antibody detected the KIS protein (arrow).| Product Name | KIST (KIS) Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | UHMK1; KIS; KIST; Serine/threonine-protein kinase Kist; Kinase interacting with stathmin; U2AF homology motif kinase 1 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | KIS KIST |
|---|---|
| Alternative Names | UHMK1 KIS KIST Serine/threonine-protein kinase Kist Kinase interacting with stathmin U2AF homology motif kinase 1 |
| Molecular Weight(MW) | 46546 Da |
| Function | Upon serum stimulation, phosphorylates CDKN1B/p27Kip1, thus controlling CDKN1B subcellular location and cell cycle progression in G1 phase. May be involved in trafficking and/or processing of RNA (By similarity) |
| Tissue Specificity | Widely expressed, with highest levels in skeletal muscle, kidney, placenta and peripheral blood leukocytes |
| Cellular Localization | Nucleus. Note=Mostly nuclear (By similarity) |
| Entrez Gene | 127933 |
|---|
Application
Western blot analysis of anti-KIS Pab in mouse heart tissue lysate. KIS (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
Application
KIS Antibody (C6) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the KIS antibody detected the KIS protein (arrow).| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
