Application
Flow cytometric analysis of MCF-7 cells using KLF4 Monoclonal Antibody (bottom histogram) compared to a negative control cell (top histogram). PE-conjugated goat-anti-mouse secondary antibodies were used for the analysis.| Product Name | KLF4 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | KLF4; EZF; GKLF; Krueppel-like factor 4; Epithelial zinc finger protein EZF; Gut-enriched krueppel-like factor |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 56CT5.1.6 |
| Host Species | Mouse |
| Tested Applications | FC |
| FC:1:10~50 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | EZF GKLF |
|---|---|
| Alternative Names | KLF4 EZF GKLF Krueppel-like factor 4 Epithelial zinc finger protein EZF Gut-enriched krueppel-like factor |
| Molecular Weight(MW) | 54671 Da |
| Function | Transcription factor; can act both as activator and as repressor. Binds the 5'-CACCC-3' core sequence. Binds to the promoter region of its own gene and can activate its own transcription. Regulates the expression of key transcription factors during embryonic development. Plays an important role in maintaining embryonic stem cells, and in preventing their differentiation. Required for establishing the barrier function of the skin and for postnatal maturation and maintenance of the ocular surface. Involved in the differentiation of epithelial cells and may also function in skeletal and kidney development Contributes to the down-regulation of p53/TP53 transcription |
| Tissue Specificity | KLF4 recombinant protein is used to produce this monoclonal antibody. |
| Cellular Localization | Nucleus. |
| Entrez Gene | 9314 |
|---|
Application
Flow cytometric analysis of MCF-7 cells using KLF4 Monoclonal Antibody (bottom histogram) compared to a negative control cell (top histogram). PE-conjugated goat-anti-mouse secondary antibodies were used for the analysis.| Application Notes | FC:1:10~50 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified mouse monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
