Application
KLH20 Antibody (C-term) western blot analysis in U251,NCI-H460,K562 cell line lysates (35ug/lane).This demonstrates the KLH20 antibody detected the KLH20 protein (arrow).Application
KLH20 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the KLH20 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.| Product Name | KLH20 Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | KLHL20; KLEIP; Kelch-like protein 20; Kelch-like ECT2-interacting protein; Kelch-like protein X |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | KLEIP |
|---|---|
| Alternative Names | KLHL20 KLEIP Kelch-like protein 20 Kelch-like ECT2-interacting protein Kelch-like protein X |
| Molecular Weight(MW) | 67955 Da |
| Function | Substrate-specific adapter of a BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complex involved in interferon response The BCR(KLHL20) E3 ubiquitin ligase complex mediates the ubiquitination of DAPK1, leading to its degradation by the proteasome, thereby acting as a negative regulator of apoptosis Also acts as a regulator of endothelial migration during angiogenesis by controlling the activation of Rho GTPases |
| Tissue Specificity | This KLH20 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 419-448 amino acids from the C-terminal region of human KLH20. |
| Cellular Localization | Cytoplasm, perinuclear region. Nucleus. Note=Localizes in the perinuclear region in normal conditions Following IFN-alpha or IFN-gamma treatment, it is relocalized and sequestrated to the PML nuclear bodies, preventing DAPK1 ubiquitination |
| Entrez Gene | 27252 |
|---|---|
| Nucleotide Accession | NP_055273.2 |
Application
KLH20 Antibody (C-term) western blot analysis in U251,NCI-H460,K562 cell line lysates (35ug/lane).This demonstrates the KLH20 antibody detected the KLH20 protein (arrow).
Application
KLH20 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the KLH20 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
