Application
KLHL12 Antibody (C-term) western blot analysis in mouse NIH-3T3 cell line lysates (35ug/lane).This demonstrates the KLHL12 antibody detected the KLHL12 protein (arrow).Application
KLHL12 Antibody (C-term) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the KLHL12 antibody detected the KLHL12 protein (arrow).| Product Name | KLHL12 Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | KLHL12; C3IP1; Kelch-like protein 12; CUL3-interacting protein 1; DKIR homolog |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:100~500 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | C3IP1 |
|---|---|
| Alternative Names | KLHL12 C3IP1 Kelch-like protein 12 CUL3-interacting protein 1 DKIR homolog |
| Molecular Weight(MW) | 63277 Da |
| Function | Substrate-specific adapter of a BCR (BTB-CUL3-RBX1) E3 ubiquitin ligase complex that acts as a negative regulator of Wnt signaling pathway and ER-Golgi transport. The BCR(KLHL12) complex is involved in ER-Golgi transport by regulating the size of COPII coats, thereby playing a key role in collagen export, which is required for embryonic stem (ES) cells division: BCR(KLHL12) acts by mediating monoubiquitination of SEC31 (SEC31A or SEC31B). As part of the BCR(KLHL12) complex, also acts as a negative regulator of the Wnt signaling pathway by mediating ubiquitination and subsequent proteolysis of DVL3. The BCR(KLHL12) complex also mediates polyubiquitination of DRD4, without leading to degradation of DRD4 |
| Tissue Specificity | Ubiquitously expressed. Highly expressed in testis and at lower levels in the submandibular salivary gland |
| Cellular Localization | Cytoplasmic vesicle, COPII-coated vesicle. |
| Entrez Gene | 59349 |
|---|---|
| Nucleotide Accession | NP_067646.1 |
Application
KLHL12 Antibody (C-term) western blot analysis in mouse NIH-3T3 cell line lysates (35ug/lane).This demonstrates the KLHL12 antibody detected the KLHL12 protein (arrow).
Application
KLHL12 Antibody (C-term) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the KLHL12 antibody detected the KLHL12 protein (arrow).| Application Notes | WB:1:100~500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
