Application
KLHL13 Antibody (N-term ) western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the KLHL13 antibody detected the KLHL13 protein (arrow).Application
KLHL13 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of KLHL13 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.Application
KLHL13 Antibody (N-term ) flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.| Product Name | KLHL13 Antibody (N-term ) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | KLHL13; BKLHD2; KIAA1309; Kelch-like protein 13; BTB and kelch domain-containing protein 2 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | BKLHD2 KIAA1309 |
|---|---|
| Alternative Names | KLHL13 BKLHD2 KIAA1309 Kelch-like protein 13 BTB and kelch domain-containing protein 2 |
| Molecular Weight(MW) | 73868 Da |
| Function | Substrate-specific adapter of a BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complex required for mitotic progression and cytokinesis. The BCR(KLHL9-KLHL13) E3 ubiquitin ligase complex mediates the ubiquitination of AURKB and controls the dynamic behavior of AURKB on mitotic chromosomes and thereby coordinates faithful mitotic progression and completion of cytokinesis |
| Tissue Specificity | This KLHL13 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 79-109 amino acids from the N-terminal region of human KLHL13. |
| Entrez Gene | 90293 |
|---|---|
| Nucleotide Accession | NP_001161772.1 NP_001161774.1 NP_001161773.1 |
Application
KLHL13 Antibody (N-term ) western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the KLHL13 antibody detected the KLHL13 protein (arrow).
Application
KLHL13 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of KLHL13 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
KLHL13 Antibody (N-term ) flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
