Application
KRAS Antibody (C-term) western blot analysis in mouse NIH-3T3 cell line lysates (35ug/lane).This demonstrates the KRAS antibody detected the KRAS protein (arrow).Application
Confocal immunofluorescent analysis of KRAS Antibody (C-term)(Cat#AP16356b) with WiDr cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).Application
KRAS Antibody (C-term) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | KRAS Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | KRAS; KRAS2; RASK2; GTPase KRas; K-Ras 2; Ki-Ras; c-K-ras; c-Ki-ras; GTPase KRas, N-terminally processed |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIFFC |
| WB:1:100~500 IF |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | KRAS2 RASK2 |
|---|---|
| Alternative Names | KRAS KRAS2 RASK2 GTPase KRas K-Ras 2 Ki-Ras c-K-ras c-Ki-ras GTPase KRas N-terminally processed |
| Molecular Weight(MW) | 21656 Da |
| Function | Ras proteins bind GDP/GTP and possess intrinsic GTPase activity |
| Tissue Specificity | This KRAS antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 145-174 amino acids from the C-terminal region of human KRAS. |
| Cellular Localization | Cell membrane; Lipid-anchor; Cytoplasmic side |
| Entrez Gene | 3845 |
|---|---|
| Nucleotide Accession | NP_203524.1 NP_004976.2 |
Application
KRAS Antibody (C-term) western blot analysis in mouse NIH-3T3 cell line lysates (35ug/lane).This demonstrates the KRAS antibody detected the KRAS protein (arrow).
Application
Confocal immunofluorescent analysis of KRAS Antibody (C-term)(Cat#AP16356b) with WiDr cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).
Application
KRAS Antibody (C-term) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 IF |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
