Application
Western blot analysis of KRT19 antibody (Center) (Cat.# AP2823c) in MCF-7 cell line lysates (35ug/lane). KRT19 (arrow) was detected using the purified Pab.Application
KRT19 Antibody (Center) (Cat.# AP2823c) IHC analysis in formalin fixed and paraffin embedded human Lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the KRT19 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.Application
KRT19 Antibody (Center) flow cytometric analysis of MCF-7 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Western blot analysis of KRT19 (arrow) using rabbit polyclonal KRT19 Antibody (Center) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the KRT19 gene.| Product Name | KRT19 Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | KRT19; Keratin, type I cytoskeletal 19; Cytokeratin-19; Keratin-19 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:50~100 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | KRT19 Keratin type I cytoskeletal 19 Cytokeratin-19 Keratin-19 |
|---|---|
| Molecular Weight(MW) | 44106 Da |
| Function | Involved in the organization of myofibers. Together with KRT8, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle |
| Tissue Specificity | Expressed in a defined zone of basal keratinocytes in the deep outer root sheath of hair follicles Also observed in sweat gland and mammary gland ductal and secretory cells, bile ducts, gastrointestinal tract, bladder urothelium, oral epithelia, esophagus, ectocervical epithelium (at protein level). Expressed in epidermal basal cells, in nipple epidermis and a defined region of the hair follicle. Also seen in a subset of vascular wall cells in both the veins and artery of human umbilical cord, and in umbilical cord vascular smooth muscle. Observed in muscle fibers accumulating in the costameres of myoplasm at the sarcolemma in structures that contain dystrophin and spectrin |
| Entrez Gene | 3880 |
|---|
Application
Western blot analysis of KRT19 antibody (Center) (Cat.# AP2823c) in MCF-7 cell line lysates (35ug/lane). KRT19 (arrow) was detected using the purified Pab.
Application
KRT19 Antibody (Center) (Cat.# AP2823c) IHC analysis in formalin fixed and paraffin embedded human Lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the KRT19 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
KRT19 Antibody (Center) flow cytometric analysis of MCF-7 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Western blot analysis of KRT19 (arrow) using rabbit polyclonal KRT19 Antibody (Center) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the KRT19 gene.| Application Notes | WB:1:50~100 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
