Application
Western blot analysis of KRT27 Antibody (C-term) in MDA-MB435 cell line lysates (35ug/lane).KRT27 (arrow) was detected using the purified Pab.Application
KRT27 Antibody (C-term) IHC analysis in formalin fixed and paraffin embedded human breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the KRT27 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.| Product Name | KRT27 Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | KRT27; KRT25C; Keratin, type I cytoskeletal 27; Cytokeratin-27; Keratin-25C; Keratin-27; Type I inner root sheath-specific keratin-K25irs3 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | KRT25C |
|---|---|
| Alternative Names | KRT27 KRT25C Keratin type I cytoskeletal 27 Cytokeratin-27 Keratin-25C Keratin-27 Type I inner root sheath-specific keratin-K25irs3 |
| Molecular Weight(MW) | 49822 Da |
| Function | Essential for the proper assembly of type I and type II keratin protein complexes and formation of keratin intermediate filaments in the inner root sheath (irs) (By similarity) |
| Tissue Specificity | Strongly expressed in skin and scalp. In the hair follicle, expressed in Henle layer, Huxley layer and in the inner root sheath cuticle of the hair follicle. Expression extends from the bulb region up to the point of differentiation into the three layers. Also present in the medulla of beard hair (at protein level) |
| Cellular Localization | Cytoplasm (By similarity). |
| Entrez Gene | 342574 |
|---|
Application
Western blot analysis of KRT27 Antibody (C-term) in MDA-MB435 cell line lysates (35ug/lane).KRT27 (arrow) was detected using the purified Pab.
Application
KRT27 Antibody (C-term) IHC analysis in formalin fixed and paraffin embedded human breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the KRT27 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
