Application
KSR2 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of KSR2 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.Application
KSR2 Antibody (C-term) western blot analysis in 293,K562,HepG2,Hela cell line lysates (35ug/lane).This demonstrates the KSR2 antibody detected the KSR2 protein (arrow).Application
KSR2 Antibody (C-term) flow cytometric analysis of MCF-7 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | KSR2 Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | KSR2; Kinase suppressor of Ras 2 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHCWBFC |
| IHC:1:50~100 WB |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | KSR2 Kinase suppressor of Ras 2 |
|---|---|
| Molecular Weight(MW) | 107632 Da |
| Function | Location-regulated scaffold connecting MEK to RAF. Blocks MAP3K8 kinase activity and MAP3K8-mediated signaling. Acts as a negative regulator of MAP3K3-mediated activation of ERK, JNK and NF-kappa-B pathways, inhibiting MAP3K3-mediated interleukin-8 production |
| Tissue Specificity | Mainly expressed in brain and kidney. |
| Cellular Localization | Cytoplasm (By similarity). Membrane; Peripheral membrane protein (By similarity) |
| Entrez Gene | 283455 |
|---|---|
| Nucleotide Accession | NP_775869.3 |
Application
KSR2 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of KSR2 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
KSR2 Antibody (C-term) western blot analysis in 293,K562,HepG2,Hela cell line lysates (35ug/lane).This demonstrates the KSR2 antibody detected the KSR2 protein (arrow).
Application
KSR2 Antibody (C-term) flow cytometric analysis of MCF-7 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | IHC:1:50~100 WB |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
