Application
Western blot analysis on (A) HeLa, (B) 293T, (C) A549, and (D) A431 cell lysates using anti-Ku70 RabMAb (Cat. #AJ1430a).Application
Immunohistochemical staining of paraffin-embedded human testis tissue using anti-Ku70 RabMAb (Cat. #AJ1430a).Application
Immunohistochemical staining of paraffin-embedded human tonsil tissue using anti-Ku70 RabMAb (Cat. #AJ1430a).Application
Immunofluorescent staining of HeLa cells using anti-Ku70 RabMAb (Cat. #AJ1430a).| Product Name | Ku70 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Monoclonal |
|---|---|
| Clone Number | EPR4027 |
| Host Species | Rabbit |
| Tested Applications | WBIHCIF |
| WB:1:2000~10000 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | G22P1 |
|---|---|
| Molecular Weight(MW) | 69843 Da |
| Function | Single stranded DNA-dependent ATP-dependent helicase. Has a role in chromosome translocation. The DNA helicase II complex binds preferentially to fork-like ends of double-stranded DNA in a cell cycle-dependent manner. It works in the 3'-5' direction. Binding to DNA may be mediated by XRCC6. Involved in DNA non-homologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination. The XRCC5/6 dimer acts as regulatory subunit of the DNA-dependent protein kinase complex DNA-PK by increasing the affinity of the catalytic subunit PRKDC to DNA by 100-fold. The XRCC5/6 dimer is probably involved in stabilizing broken DNA ends and bringing them together. The assembly of the DNA-PK complex to DNA ends is required for the NHEJ ligation step. Required for osteocalcin gene expression Probably also acts as a 5'-deoxyribose-5-phosphate lyase (5'-dRP lyase), by catalyzing the beta-elimination of the 5' deoxyribose- 5-phosphate at an abasic site near double-strand breaks. 5'-dRP lyase activity allows to 'clean' the termini of abasic sites, a class of nucleotide damage commonly associated with strand breaks, before such broken ends can be joined. The XRCC5/6 dimer together with APEX1 acts as a negative regulator of transcription |
| Tissue Specificity | A synthetic peptide corresponding to residues in human Ku70 was used as an immunogen. |
| Cellular Localization | Nucleus. Chromosome. |
| Entrez Gene | 2547 |
|---|
Application
Western blot analysis on (A) HeLa, (B) 293T, (C) A549, and (D) A431 cell lysates using anti-Ku70 RabMAb (Cat. #AJ1430a).
Application
Immunohistochemical staining of paraffin-embedded human testis tissue using anti-Ku70 RabMAb (Cat. #AJ1430a).
Application
Immunohistochemical staining of paraffin-embedded human tonsil tissue using anti-Ku70 RabMAb (Cat. #AJ1430a).
Application
Immunofluorescent staining of HeLa cells using anti-Ku70 RabMAb (Cat. #AJ1430a).| Application Notes | WB:1:2000~10000 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
