Application
Western blot analysis of LAMP2 (arrow) using LAMP2 Antibody (Center) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the LAMP2 gene (Lane 2) (Origene Technologies). Mature, functional LAMP2 is extensively glycosylated with a variety of different N linked and O linked oligosaccharides with a total molecular weight of ~100-110 kDa.| Product Name | LAMP2 Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | LAMP2; Lysosome-associated membrane glycoprotein 2; CD107 antigen-like family member B; CD_antigen=CD107b; Flags: Precursor |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:50~100 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | LAMP2 Lysosome-associated membrane glycoprotein 2 CD107 antigen-like family member B CD_antigen=CD107b Flags: Precursor |
|---|---|
| Molecular Weight(MW) | 44961 Da |
| Function | Implicated in tumor cell metastasis. May function in protection of the lysosomal membrane from autodigestion, maintenance of the acidic environment of the lysosome, adhesion when expressed on the cell surface (plasma membrane), and inter- and intracellular signal transduction. Protects cells from the toxic effects of methylating mutagens |
| Tissue Specificity | Isoform LAMP-2A is highly expressed in placenta, lung and liver, less in kidney and pancreas, low in brain and skeletal muscle. Isoform LAMP-2B is highly expressed in skeletal muscle, less in brain, placenta, lung, kidney and pancreas, very low in liver |
| Cellular Localization | Cell membrane; Single-pass type I membrane protein. Endosome membrane; Single-pass type I membrane protein Lysosome membrane; Single-pass type I membrane protein. Note=This protein shuttles between lysosomes, endosomes, and the plasma membrane |
| Entrez Gene | 3920 |
|---|
Application
Western blot analysis of LAMP2 (arrow) using LAMP2 Antibody (Center) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the LAMP2 gene (Lane 2) (Origene Technologies). Mature, functional LAMP2 is extensively glycosylated with a variety of different N linked and O linked oligosaccharides with a total molecular weight of ~100-110 kDa.| Application Notes | WB:1:50~100 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified rabbit polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
